Table 1.
List of oligonucleotides used for PCR amplifications.
| Target Transcript (Use) |
Sequence (Sense/Antisense Primers) | Amplicon Length (bp) |
|---|---|---|
| β-actin (qPCR) |
5′-GAA ACT ACC TTC AAC TCC ATC-3′/ 5′-GAA ACT ACA TTC AAT TCC ATC-3′ |
170 |
| PEDF-R (qPCR) |
5′-TGT GGC CTC ATT CCT CCT AC-3′/ 5′-CAA GTT GTC TGA AAT GCC G-3′ |
66 |
| PEDF-R (RT-PCR) |
5′-AGA GAT GTG CAA GCA GGG-3′/ 5′-GCA CAG GCA GCA TGT TGG-3′ |
119 (RGC), 263 (R28), 281 (RPE cells, ARPE-19) |
| LR (qPCR) |
5′-ACA TCA TAA ACC TGA AGA GGA C-3′/ 5′-TGG ATC TGG TTA GTG AAG GT-3′ |
199 |
| LR (RT-PCR) |
5′-ACATCATAAACCTGAAGAGGAC-3′/ 5′-TGGATCTGGTTAGTGAAGGT-3′ |
199 (RGC) |
| LR (RT-PCR) |
5′-GGAACCCACTTAGGTGGCA-3′/ 5′-TGGATCTGGTTAGTGAAGGT-3′ |
275 (R28, RPE cells, ARPE-19) |
| VEGF (qPCR) |
5′-AGA AAG CCC ATG AAG TGG TG-3′/ 5′-ACT CCA GGG CTT CAT CAT TG-3′ |
177 |
| PEDF (qPCR) |
5′-GAC ATG AAG CTA CAG TCC TTG T-3′/ 5′-CCC TCC TCA TTC CAC TCA AA-3′ |
113 |
| BDNF (qPCR) |
5′-ATGACCATCCTTTTCCTTAC-3′/ 5′-TCACGTGCTCAAAAGTGTCA-3′ |
205 |
| Bcl-2 (qPCR) |
5′-CTG GGA TGC CTT TGT GGA A-3′/ 5′-CAG AGA CAG CCA GGA GAA ATC A-3′ |
70 |
| Bcl-xL (qPCR) |
5′-ATG TCT CAG AGC AAC CGG GA-3′/ 5′-CAG GAT GGG TTG CCA TTG AT-3′ |
170 |