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. 2021 Jan 2;22(1):406. doi: 10.3390/ijms22010406

Figure 2.

Figure 2

Activation of the AKT- IκB kinase (IKK)-inhibitors of NF-κB (IκB) pathway is associated with the CK2 down-regulation-mediated nuclear import of NF-κB. (A) MCF-7 and HCT116 cells were transfected with CK2α siRNA or pcDNA3.1-HA-CK2α for two days. The level of each protein was determined by immunoblot analysis using specific antibodies (left). Representative data from three independent experiments are shown. β-Actin was used as a control. Graphs represent the quantitation of p-AKT, IKK, and IκBα relative to β-actin and that of p-IKK and p-IκBα relative to the unphosphorylated proteins (right). (B) Cells were transfected with CK2α siRNA or pcDNA3.1-HA-CK2α for two days in the absence or presence of 1 μM triciribine (TCN). Cytoplasm and nuclei were isolated from cells, and both extracts were visualized by immunoblotting. α-Tubulin (cytoplasmic marker) and histone H3 (nuclear marker) were quantified as loading controls (left). Representative data from three independent experiments are shown. Graphs represent the quantitation of RelA/p65 relative to the subcellular markers (right). exp, exposure. Data are mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001.