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. 2021 Jan 2;22(1):406. doi: 10.3390/ijms22010406

Figure 3.

Figure 3

SIRT1 attenuates both RelA/p65 acetylation and activation of the AKT-IKK-IκB axis mediated by CK2 down-regulation. (A) MCF-7 and HCT116 cells were transfected with CK2α siRNA or pcDNA3.1-HA-CK2α for two days in the absence or presence of 20 μM resveratrol or 15 mM nicotine amide. The level of each protein was determined by immunoblot analysis using specific antibodies (top). Representative data from three independent experiments are shown. β-Actin was used as a control. Graphs represent the quantitation of acetylated p65 (ac-p65) relative to unacetylated p-65 (bottom). RSV, resveratrol; NA, nicotine amide. (B) Cells were transfected with CK2α siRNA and/or pECE-Flag-SIRT1 for two days. The level of each protein was determined by immunoblot analysis using specific antibodies (top). Representative data from three independent experiments are shown. β-Actin was used as a control. Graphs represent the quantitation of p-AKT, IKK, and IκBα relative to β-actin and that of p-IKK and p-IκBα relative to unphosphorylated proteins (bottom). Data are mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001.