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. 2021 Jan 2;22(1):406. doi: 10.3390/ijms22010406

Figure 5.

Figure 5

Antisense inhibitors of miR-186, miR-216b, miR-337-3p, and miR-760 attenuate the induction of senescence-associated secretory phenotype (SASP) factors by lipopolysaccharide (LPS). (A) MCF-7 and HCT116 cells were transfected with the 4 miRs (miR-186, miR-216b, miR-337-3p, and miR-760) or the 4 miRi. (B,C) Cells were treated with LPS (6 μg/μL) in the presence or absence of the 4 miRi or pcDNA3.1-HA-CK2α for 2 days. The level of each protein was determined by immunoblot analysis using specific antibodies (left). Representative data from three independent experiments are shown. β-Actin was used as a control. Graphs represent the quantitation of p-AKT, IKK, and IκBα relative to β-actin and that of p-IKK and p-IκBα relative to the unphosphorylated proteins (right). Data are mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001.