Figure 4.

BAG regulates the generation of RANKL-induced ROS and the expression of antioxidant genes. (A) Representative stain images of RANKL-induced ROS generation detected by DCFH-DA (green) and immunofluorescence for nuclei using DAPI (blue) in RAW264.7 with different indicated concentrations of BAG. (B) The expressions of SOD and CAT antioxidant proteins were analyzed by Western blot analysis from cells pre-treated with BAG and stimulated by RANKL (50 ng/mL). (C) The mRNA level of antioxidant genes was measured by real-time PCR. * p < 0.05, versus the RANKL treated group.