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. 2020 Dec 28;22(1):222. doi: 10.3390/ijms22010222

Figure 5.

Figure 5

BAG downregulates RANKL-induced expression of osteoclast specific protein and gene. (A) For total proteins from RAW264.7 stimulated with RANKL without or with indicated concentration for 24 h, Western blot analyses were performed using specific antibodies against c-Fos and NFATc1. The protein expression of c-Fos and NFATc1 relative to b-actin were quantified by densitometry. (B) The mRNA level of osteoclast-specific genes was measured by real-time PCR. * p < 0.05 versus the RANKL-treated group.