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Flowchart of HER2 testing of molecularly classified PORTEC-3 trial participants. * SISH was performed on a random selection of cases with absent (n = 10) and faint (n = 10) membranous staining to confirm the absence of HER2 amplification. Abbreviations: IHC, immunohistochemistry; SISH, silver in situ hybridization; NGS, next-generation sequencing.
