Figure 8.

Fenofibric acid did not interfere with paclitaxel-induced cytotoxicity in breast tumor cells. (a,b) Cell viability: 4T1 cells were exposed to fenofibric acid (FA, 20 or 40 μM), paclitaxel (Pac, 50 nM) or a combination of both for 24 h. The number of viable cells was monitored over 7 days via Trypan blue exclusion. Cell viability over time was graphed for all conditions (a) and paclitaxel-treated conditions (b) (n = 3). (c) Clonogenic survival cells were exposed to fenofibric acid (20 or 40 μM), paclitaxel (50 nM) or a combination for 24 h. Cells were allowed to grow for ~11 days with fresh medium, and colonies were stained and counted (n = 3). (d) Extent of apoptosis: cells were stained with annexin-V and propidium iodide (PI) and quantified using flow cytometry to assess the extent of apoptotic cells following 48 h treatment with fenofibric acid (20 or 40 μM), paclitaxel (50 nM) or a combination for 24 h (n = 3). Results are from three independent experiments. * p < 0.05, **** p < 0.0001 compared to controls. Not significant (n.s.) compared to paclitaxel treated groups.