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. 2020 Dec 29;22(1):271. doi: 10.3390/ijms22010271

Figure 2.

Figure 2

Cyclophilin expression, renal function and tubular damage in renal ischemia/reperfusion injury (IRI). Groups of mice (n = 10) were treated with vehicle, 10 or 30 mg/kg/BID GS-642462 and compared to sham controls (n = 6). RT-PCR for mRNA levels of (A) CypA and (B) CypD. (C) Plasma creatinine levels. (D–G) Periodic-acid-Schiff-stained kidney sections. (D) Sham operated showing normal kidney structure. (E) Vehicle-treated IRI showing severe tubular damage in the outer medulla featuring tubular cell loss/sloughing and cast formation. (F) IRI treated with 10 mg/kg GS-642362 shows extensive tubular damage. (G) IRI treated with 30 mg/kg GS-642362 shows lesser, but still significant, tubular damage. Bar = 200 µm. (H) Graph of tubular damage. (I) RT-PCR for mRNA levels of the tubular damage marker, NGAL/LCN2.