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. 2020 Dec 31;22(1):378. doi: 10.3390/ijms22010378

Figure 3.

Figure 3

Effect of coiled coil 1 (CC1) interhelical contacts on stromal interaction molecule 1 (STIM1) gain-of-function (GOF) mutations. (a) Patch clamp recordings showing current activation (current density, pA/pF) of N-terminally tagged CFP–Orai1 co-expressed with YFP–STIM1 R304W ± I290S + A293S. Adapted from [22]. (b) Same as in (a), but for YFP–STIM1 C227W ± I290S + A293S. (c) Same as in (a), but for YFP–STIM1 D247C ± I290S + A293S. (d) Comparison of current activation levels at Time 0. HEK293 cells were used for all experiments. Student’s two-tailed t-test was employed for statistical analyses with differences considered statistically significant at p < 0.05. Asterisks (*) indicate significant difference (n.s., not significant). Data represent mean values ± SEM.