Figure 3.

Similarity in the colony-forming potential of DP-MSCs from OP and HC. Equal cell numbers from OP and HC subjects were seeded at a density of approximately 500 cells/well and incubated at 37 °C for 10 days. Cells were washed and subsequently stained with 0.1% crystal violet (Santa Cruz) for visualization. Individual samples in each of the analyzed cohort were considered as biological replicates of that group, while at least duplicates runs of each of the pairs of OP and HC were considered as analytical replicates in order to demonstrate the reproducibility of the colony forming potentials of the two cell types from OP and HC. The histogram shows the quantitation of staining as visualized under the microscope. The average intensity of each of the 6 wells was used to generate the histogram. The variation was very minimal and had no statistically significant difference (p > 0.05), so we used stack plots rather than individual bars. The y-axis shows the quantitation as a measure of the optical density of the scanned wells, and the x-axis gives the number of replicated cell cultures.