Table 2.
Some of the 86 proteins in the dataset that were significantly differentially expressed between the bulk undifferentiated HC and OP samples were mapped in Ingenuity Pathway Analysis (IPA). Their functional annotations are indicated, as are their involvements in osteogenesis as well as in different bone and connective tissue disorders.
| Symbol | Entrez Gene Name | GenPept/ UniProt/Swiss-Prot Accession |
Expr Fold Change OP:HC | Location | Family | Bone Involvement |
|---|---|---|---|---|---|---|
| COL1A1 | Collagen type I alpha 1 chain | P02452 | −2.26587705 | Extracellular Space | Other | Bone matrix/Osteoblast |
| COL3A1 | Collagen type III alpha 1 chain | P02461 | −6.1868361 | Extracellular Space | Other | Bone matrix/Osteoblast |
| FERMT2 | Fermitin family member 2 | Q96AC1 | −6.11159085 | Cytoplasm | Other | Bone homeostasis regulation |
| GLB1 | Galactosidase beta 1 | P16278 | 2.92715725 | Cytoplasm | Enzyme | |
| GNAI3 | G protein subunit alpha i3 | P08754 | −2.52033996 | Cytoplasm | Enzyme | Impaired activation of integrin and ERK1/2. |
| HADH | Hydroxyacyl-CoA dehydrogenase | Q16836 | 2.1108754 | Cytoplasm | Enzyme | In OP gene panel |
| ITGA5 | Integrin subunit alpha 5 | P08648 | 2.88383695 | Plasma Membrane | Transmembrane Receptor | osteoclastogenesis |
| KDM3A | Lysine demethylase 3A | Q9Y4C1 | 3.43441641 | Nucleus | Transcription regulator | OP associated |
| MAPK3 | Mitogen-activated protein kinase 3 | P27361 | 25.6856293 | Cytoplasm | Kinase | Bone Diseases |
| PCBP2 | Poly(rC) binding protein 2 | Q15366 | 2.05142965 | Nucleus | Other | |
| PCOLCE | Procollagen C-endopeptidase enhancer | Q15113 | 3.37138722 | Extracellular Space | Other | osteoclast–osteoblast bone modeling |
| PLOD2 | Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 | O00469 | 2.26356027 | Cytoplasm | Enzyme | bone mass syndromes |
| PTPN12 | Protein tyrosine phosphatase non-receptor type 12 | Q05209 | 2.40278726 | Cytoplasm | Phosphatase | Bone resorbing |
| S100A4 | S100 calcium binding protein A4 | P26447 | 2.45896457 | Cytoplasm | Other | Bone resorption osteoclast function |
| YBX1 | Y-box binding protein 1 | P67809 | 2.57704761 | Nucleus | Transcription regulator |
The fold change was calculated based on the expression values of OP/HC. Therefore, the positive fold-change values mean upregulation in OP compared with HC and vice versa for negative values, i.e., upregulated in HC compared with OP.