Figure 1.

Cytotoxic effect of phenols on leukemic cells. (a–f) Cytotoxic effect of phenolic compounds was estimated by means of resazurin-based metabolic assay. T-ALL cells (Jurkat) were incubated in the presence or absence of aspirin (a), cannabidiol, CBD (b), chlorogenic acid (c), curcumin (d), gallic acid (e), methyl gallate (f), protocatechuic acid (g) and quercetin (h), for 24 h. Resorufin fluorescence was measured and normalized to untreated cells. Data are mean ± SD (n = 9 from three independent experiments; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; one-way ANOVA). Non-linear fit of the dose-dependence yields the following IC50 values (in µM): 12.1 for CBD and 36.5 for curcumin.