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. 2021 Jan 2;22(1):405. doi: 10.3390/ijms22010405

Figure 7.

Figure 7

P2X3-dependent nocifensive behavior is altered in Slack-/- mice. Paw-licking responses induced by paw injection of drugs are shown. (A) The immediate paw licking in the first 2 min after injection of α,β-meATP (12 nmol) is increased in naive Slack-/- mice compared with WT mice (n = 7/genotype; p = 0.0398 in 0–2 min; p = 0.9800 in 2–6 min; p = 0.9352 in 6–10 min). (B) No significant differences between groups occurred when the P2X3 receptor antagonist AF353 (70 nmol intraplantar) was injected 10 min prior to α,β-meATP (n = 5–7/genotype; p = 0.9664 in 0–2 min; p = 0.9732 in 2–6 min; p = 0.7546 in 6–10 min). (C) Paw-licking responses after injection of the vehicle were comparable in Slack-/- and WT mice (n = 6/genotype; p = 0.9999 in 0–2 min; p = 0.8934 in 2–6 min; p = 0.8709 in 6–10 min) and similar to the licking behavior after combined injection of α,β-meATP and AF353 (B). (D) When α,β-meATP (12 nmol) was injected in the ipsilateral hind paw after SNI, the paw-licking response persisted over the 10 min observation period in WT mice. In Slack-/- mice, the paw licking was increased in the first 2 min and decreased from 6–10 min compared with WT mice (n = 16/genotype; p = 0.0262 in 0–2 min; p = 0.9993 in 2–6 min; and p = 0.0362 in 6–10 min). Two-way ANOVA tests were performed. Bars denote mean ± SEM. * p ˂ 0.05.