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Primary antibodies and their conditions of use.
| Monoclonal Antibody | Reference | Dilution | Incubation Time and Temperature |
|---|---|---|---|
| Anti-VEGF | Clone VG1 (Novus Biologicals) | 1:50 | 60’ at RT |
| Anti-VEGFR1/Flt-1 | Clone CL0345 (Novus Biologicals) | 1:200 | 60’at RT |
| Anti-VEGFR2/KDR/Flk-1 | Clone EIC (Novus Biologicals) | 1:10 | 120’ at RT plus 4 °C overnight |
RT—Room Temperature.
