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. 2020 Dec 30;22(1):293. doi: 10.3390/ijms22010293

Figure 7.

Figure 7

(a) OCR was measured in wild-type and mutant strains grown at 37 °C in SC medium supplemented with 0.6% glucose. Values are means ± standard deviation. The OCR was normalized to the strain transformed with the CAB1 wild-type allele. Values are means ± standard deviation. N = 3; n = 9 for each strain. **: p < 0.01; ns: not significant. (b) Steady state level of Cab1 protein in wild-type and mutant strains. The proteins were extracted from wild-type and mutant strains grown at 37 °C, or 28 °C for cab1N290I, in SC medium supplement with 0.6% glucose. Quantification of protein bands was performed using the Image Lab software. N = 2; n = 2 for each strain. *: p < 0.05; **: p < 0.01; ns: not significant. (c) Equal amounts of serial dilutions of wild-type and mutant strains from exponentially grown cultures were spotted (104, 103, 102, 101 cell/spot) onto SC medium plates supplemented with 2% lactate, 2% lactate with DMSO and 2% lactate with 31 µM nalH. The growth was scored after 4 days of incubation at 37 °C. The experiment was performed on at least three independent clones for each strain (N = 2; n = 6).