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. 2020 Dec 24;22(1):123. doi: 10.3390/ijms22010123

Figure 2.

Figure 2

Immunofluorescence analysis of tight junction marker Zo-1 in HMC, grown on permeable supports and exposed for 8 h to different PD solutions at the apical side. HMC monolayers grown on permeable supports were incubated for 8 h at the apical side with high osmolarity glucose-based PD solutions (Physioneal 40®, Dianeal PD4® and BicaVera®), glucose-free Extraneal®/Nutrineal® and high osmolarity glucose-sparing PD solutions (± l-carnitine). Cells were immunostained with anti Zo-1 antibodies (green) and scanned by laser confocal microscopy. Nuclei were stained with propidium iodide (red). Scale bars = 10 μm.