Figure 2.

Synthesis of cytokines from NK and NKT cells from PBMCs of different groups of patients with CML. Analysis by flow cytometry of the intracellular expression of interferon gamma (IFNγ), tumor necrosis factor alfa (TNFα) and granzyme B (GZB) in CD3- CD56+ CD16+ NK cells (A) and CD3+ CD56+ CD16+ NKT cells (B) from each group of patients and healthy donors (Healthy donors (●), patients On TKI (■), patients Off TKI who did not relapse (▲), and patients Off TKI who relapsed (▼)). Each dot corresponds to one sample and lines represent mean ± SEM. Statistical significance was calculated using one-way ANOVA and Tukey’s multiple comparisons test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.