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. 2020 Dec 28;22(1):198. doi: 10.3390/ijms22010198

Figure 4.

Figure 4

CoQ depletion induces alteration in enzyme activities and cell cycle. MCF-7 cells were cultured in complete DMEM for 96 h in the presence of 4 mM 4-nitrobenzoic acid (CoQ depleted) or vehicle (CTRL). (A) Enzyme activities (glutaminase, GLS; glutamate dehydrogenase, GDH; isocitrate dehydrogenase1,2 and 3; IDH1/2, IDH3; lactate dehydrogenase LDH; pyruvate dehydrogenase complex, PDC; α-ketoglutarate dehydrogenase, KGDC; malate dehydrogenase, MDH; malic enzyme ME) in control and CoQ depleted cells. Data refers to the percentage increase in comparison with controls. (C) Cell cycle analysis and quantification (B) by flow cytometry in control and CoQ depleted cells using propidium iodide dye staining. The column graph shows the percentage of cells in each cell cycle. Statistical analysis was performed using GraphPad Prism software. Error bars indicate the standard error of the mean (SEM). p values were obtained using the analysis of variance (ANOVA) followed by Tukey’s post hoc test. *** p ≤ 0.001.