Figure 5.

Effect of mitochondrial dysfunction on cell proliferation. (A) MCF-7 cell proliferation was assessed by MTT assay in the presence of different concentrations of 4-nitrobenzoic acid up to 96 h. (B) MCF-7 cells were cultured in complete DMEM for 96 h in the presence of 4 mM 4-nitrobenzoic acid (CoQ depleted) or vehicle (CTRL), then the cells were exposed to 100 µM tert-butyl hydroperoxide (TBH) up to 6 h. (C) Control and CoQ depleted cells were cultured in complete medium in the presence of 10 mM hexokinase (HK) inhibitor 2-deoxyglucose (2-DG). (D) Control and CoQ depleted cells were cultured in glucose free medium supplemented with dialyzed FBS and 5.5 mM galactose. (E) Control and CoQ depleted cells were cultured in a glutamine free complete medium. The cell proliferation in panels B-E was monitored by MTT assay for up to 72 h. (F) Control and CoQ depleted cells were cultured in complete medium for 24 h in the presence of different concentrations of trifluoromethyl benzyl-α-ketoglutarate (Tα-KG). Cell proliferation was assessed by MTT assay. (G) Control and CoQ depleted cells were cultured in complete medium for 24 h in the presence of different concentrations of doxorubicin and cisplatin (H) for 24 h. Cell proliferation was assessed by MTT assay. Statistical analysis was performed using GraphPad Prism software. Error bars indicate the standard error of the mean (SEM). p values were obtained using unpaired t-test with Welch’s correction. *** p ≤ 0.01.