Figure 1.

Bioinformatic identification and validation of Y-box binding protein-1 (YB-1) as a potential biomarker in HuH-7^R cells. (A) Strategy for identification of sorafenib resistance-related differentially expressed molecules in hepatocellular carcinoma (HCC). (B) Phosphorylation levels of YB-1 and total YB-1 expression in HuH-7 and HuH-7^R cells validated by immunoblotting, upper panel. Relative changes of the expression levels were quantified using GAPDH as normalization control, lower panel. (* p < 0.05; ** p < 0.01). (C) Kaplan-Meir OS curves of the YB-1 gene among 343 HCC patients in the TCGA dataset. The p value was calculated with the log-rank test (*** p < 0.001).