Figure 2.

YB-1 phosphorylation is regulated by different signaling pathways in response to sorafenib treatment in HuH-7 and HuH-7^R cells. (A) HuH-7 cells were incubated with increasing doses of sorafenib, U0126 or BI-D1870 for 12 h. (B) HuH-7^R cells were incubated with increasing doses of sorafenib, LY294002, AG1478 or sorafenib combined with LY294002 for 12 h. Cell lysates were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and subjected to immunoblotting with the indicated antibodies. Intensity was quantified using densitometry and normalized to that of GAPDH. Blots are representatives of three separate experiments.