Figure 3.

Effects of sorafenib on tumor growth and differentially expressed proteins of HuH-7 and HuH-7^R cells in xenograft models. (A) In vivo effects of sorafenib on tumor growth of HuH-7 or HuH-7^R mice. Nude mice were injected subcutaneously with HuH-7 or HuH-7^R cells. Mice were orally administered vehicle or sorafenib 30 mg/kg for 18 days. Tumor volumes at the indicated time-points were calculated and plotted (n = 5/group). p Values were determined using the t test. (ns, not significant; ** p < 0.01). (B) Changes in specific protein markers in HuH-7 and HuH-7^R tumors tissues. Paraffin-embedded sections of tumor tissues were analyzed via immunohistochemistry and stained with the indicated antibodies. Representative sections were captured at 400× magnification.