Figure 7.

YB-1 S102 phosphorylation is required for migration and invasion of HuH-7^R cells. (A) HuH-7^R cells were infected with lentiviruses containing empty vector control (EV), YB-1 wild-type (YB-1^WT) or YB-1 serine 102 to alanine mutant (YB-1^S102A) and after 24 h, lysed and analyzed via immunoblotting with the indicated antibodies, upper panel. The relative expression efficiency of WT and S102A YB-1 was quantified using GAPDH as normalization control, lower panel. (B) Wound healing assay of EV, YB-1^WT or YB-1^S102A -overexpressing HuH-7^R cells. The micrographs depict cells that migrated into the gap 0 h and 16 h after removal of the insert, upper panel. Wound recovery was quantified, lower panel. (C) Transwell invasion assay of EV, YB-1^WT or YB-1^S102A- overexpressing HuH-7^R cells. Cells in the central field of each insert were visualized via light microscopy, upper panel. Invaded cells were quantified, lower panel. p values were determined using the t test (scale bar = 50 μm; * p < 0.05; ** p < 0.01).