Inflammatory profile of breast cancer cell lines and fibroblasts after co-culture with PBMC. Scheme of experimental design (A). Gene expression of IL1A, IL6, IL10, IL17, IFNβ, NFĸB, and MMP11 in NF (n = 6) (B) and CAF (n = 6) (C) after co-culture with PBMC from healthy women (controls, C-PBMC). Gene expression of IL1A, IL6, IL10, IL17, IFNβ, NFĸB, and MMP11 in NF (n = 6) (D) and CAF (n = 24) (E) after co-culture with PBMC from breast cancer patients. Comparative of IL1A, IL6, IL10, IL17, IFNβ, NFĸB, and MMP11 gene expression in NF (F) and CAF (G) after co-culture with C-PBMC or BC-PBMC. Data represent the mean ± SD. (* p ≤ 0.05; ** p ≤ 0.01). Results of the qRT-PCR data were represented as fold expression.