Effect of ECN treatment on mechanical hyperalgesia, thermal hyperalgesia, mechanical allodynia and cold allodynia. (A) Inhibition of PSNL-induced mechanical hyperalgesia at 0, 3, 7, 10 and 14-day intervals by ECN (1 and 10 mg/kg i.p.). (B) Inhibition of PSNL-induced thermal hyperalgesia at 0, 3, 7, 10 and 14-day intervals by ECN (1 and 10 mg/kg i.p). (C) Inhibition of PSNL-induced mechanical allodynia at 0, 3, 7, 10 and 14-day intervals by ECN (1 and 10 mg/kg i.p). (D) Inhibition of PSNL-induced cold allodynia at 0, 3, 7, 10 and 14-day intervals by ECN (1 and 10 mg/kg i.p). The data were reported as the means ± S.D. Different letters meant statistically significant differences: (***) p < 0.001 indicate significant differences from the PSNL control group. (###) indicate significant differences from the normal control group. *** p < 0.001 (two-way ANOVA followed by Dunnett’s test).