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. 2021 Jan 1;26(1):181. doi: 10.3390/molecules26010181

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of ECN (10 mg/kg, i.p) on the immunohistochemical staining of mice spinal cord, showing the effect of ECN on PSNL induced expression of caspase-3 (A) and Nrf2 (B). ECN treatment reduced the expression of caspase-3 and enhanced the expression of Nrf2 (C). Black arrow heads show the expression of caspase-3 and Nrf2 in the spinal cord. (10× magnification, scale bar 50 μm). The data is presented as the mean (n = 3) ± S.D. Different letters meant statistically significant differences: (***) p < 0.001 indicate significant differences from the PSNL control group. (###) indicate significant differences from the normal control group. *** p < 0.001 (one-way ANOVA followed by Bonferroni post-test).

Effect of ECN (10 mg/kg, i.p) on the immunohistochemical staining of mice spinal cord, showing the effect of ECN on PSNL induced expression of caspase-3 (A) and Nrf2 (B). ECN treatment reduced the expression of caspase-3 and enhanced the expression of Nrf2 (C). Black arrow heads show the expression of caspase-3 and Nrf2 in the spinal cord. (10× magnification, scale bar 50 μm). The data is presented as the mean (n = 3) ± S.D. Different letters meant statistically significant differences: (***) p < 0.001 indicate significant differences from the PSNL control group. (###) indicate significant differences from the normal control group. *** p < 0.001 (one-way ANOVA followed by Bonferroni post-test).