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Gold nanoparticles (Au-NPs) can be decorated by adding the folic acid carboxyl group. Folic acid was activated by carboxyl groups by N-hydroxysuccinimide (NHS) and dicyclohexylcarbodiimide in anhydrous dimethyl sulfoxide (DMSO) and conjugation of NHS-ester activated folate to NH2-PEG3.5kDa-SH (FA-PEG-SH) in anhydrous DMSO in presence of triethylamine [19].
