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. 2021 Jan 2;22(1):413. doi: 10.3390/ijms22010413

Figure 1.

Figure 1

Purification of recombinant DDX3X132–607. (A) Schematic of human DDX3X’s domain architecture, indicating that DDX3X132–607 consists of all the major domains, except the nuclear export signal sequence. (B) Size exclusion chromatography purification (Superdex 200 Increase GL 10/300) of DDX3X132–607 demonstrating that DDX3X132–607 can be purified to homogeneity, eluting at ~14.5 mL. Y-axis represents absorbance at 280 nm while the x-axis represents elution volume. (C) SDS-PAGE indicating that the size exclusion chromatography (SEC)-purified DDX3X132–607 is monodispersed with the correct molecular weight (55.3 kDa).