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. 2021 Jan 2;22(1):413. doi: 10.3390/ijms22010413

Figure 3.

Figure 3

Interaction studies using microscale thermophoresis (MST). (A) Representative MST traces depicting the change in fluorescent migration of fluorescein-5-thiosemicarbazide (FITC)-JEV 5′ TR due to the excitation with an infra-red laser. Each green trace correlates to a different concentration of DDX3X132–607. (B) Binding data for DDX3X132–607 with JEV 5′ TR (n = 3). The red curve represents JEV 5′ and has a dissociation constant of 1.66 ± 0.21 µM (Std. error of regression = 1.25). The black trace represents that polyU (negative control) does not interact with DDX3X132–607. (C) Interaction between DDX3X132–607 and ZIKV 5′ TR (n = 3) studied using MST. The green curve represents ZIKV 5′ and has a dissociation constant of 7.05 ± 0.75 µM (Std. error of regression = 1.22). PolyU binding data included for reference (black).