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. 2021 Jan 2;22(1):413. doi: 10.3390/ijms22010413

Figure 4.

Figure 4

Helicase assays conducted using microscale thermophoresis (MST). (A) Comparing the change of fluorescent migration of a complementary DNA oligo in the presence of 5′ TR of JEV with ATP and either bovine serum albumin (BSA) or DDX3X132–607. The average signal to noise ratio is 14.8 ± 4.82 (n = 3). (B) Comparing the fluorescent migration of a complementary DNA oligo in the presence of ZIKV 5′ TR with ATP and either BSA or DDX3X132–607. The average signal to noise is 20.0 ± 5.06 (n = 3). (C) Investigating the role of ATP in DDX3X132–607 helicase activity. The average signal to noise ratio is 5.5 ± 0.59 (n = 3), suggesting that ATP is required to unwind RNA.