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. 2020 Dec 30;22(1):304. doi: 10.3390/ijms22010304

Figure 1.

Figure 1

Verification of a knock-out of the SLC35A1 gene in HEK293T cells. Genomic DNA (gDNA) and total RNA were isolated from the wild-type HEK293T cells and several stable transfectants, and either PCR (DNA) or RT-PCR (mRNA) was performed using SLC35A1 gene-specific primers. The amplified products were separated in 1.3% (w/v, mRNA) and 1.8% (w/v, gDNA) agarose gels and visualized with ethidium bromide. The clone selected for further use is indicated with an asterisk (*). A1KO, cells with an inactive SLC35A1 gene.