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. 2020 Dec 30;22(1):304. doi: 10.3390/ijms22010304

Figure 2.

Figure 2

Analysis of subcellular localization of the wild-type and disease-causing CST variants. CST-deficient HEK293T cells overexpressing recombinant CST variants were subjected to indirect immunofluorescent staining with anti-HA (red) and anti-organelle marker (green) antibodies ((A), counterstaining of the Golgi apparatus; (B), counterstaining of the ER). Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bar = 10 μm. TGN46, trans-Golgi network integral membrane protein 2. A1, SLC35A1.