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. 2020 Dec 30;22(1):304. doi: 10.3390/ijms22010304

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of cellular glycoproteins using lectin blotting. Proteins present in total cell lysates were separated by SDS-PAGE, transferred onto a nitrocellulose membrane and probed with selected biotinylated lectins followed by avidin D conjugated with alkaline phosphatase and colorimetric detection. SDS-PAGE-resolved proteins were stained with Coomassie Brilliant Blue G250 as a loading control. PNA, Arachis hypogea lectin; RCA I, Ricinus communis agglutinin I; ECL, Erythrina cristagalli lectin. A1, SLC35A1. The bands around 34 kDa strongly reactive towards RCA I observed in some lanes are most probably derived from the fetal bovine serum, in the presence of which the cells were cultured.