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. 2020 Dec 30;22(1):304. doi: 10.3390/ijms22010304

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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HPLC analysis of N-glycans released from the secreted reporter glycoprotein. N-Glycans derived from a modified SEAP overexpressed in wild-type (WT, top panel) and CST-deficient (A1KO, bottom panel) HEK293T cells were fluorescently labeled with 2-AB, purified and separated on the normal phase GlycoSep N column using HPLC. The left panel represents intact (undigested) structures, while the right panel corresponds to structures obtained upon a neuraminidase treatment. Respective glycans are expressed in glucose units. Sialylated structures are enclosed in brackets. Representative data from two independent analyses with a similar tendency are shown. A1, SLC35A1.