Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 30;22(1):304. doi: 10.3390/ijms22010304

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

HPLC analysis of N-glycans released from the secreted reporter glycoprotein. N-glycans derived from a modified SEAP overexpressed in wild-type (WT, top panel) and CST-deficient (A1KO, bottom panel) HEK293T cells were fluorescently labeled with 2-AB, purified and separated on the normal phase GlycoSep N column using HPLC. The left panel represents intact (undigested) structures; the middle panel reflects structures obtained upon a combined digestion with galactosidase and hexosaminidase; and the right panel reflects structures obtained upon a digestion with neuraminidase followed by a combined digestion with galactosidase and hexosaminidase. Respective glycans are expressed in glucose units. Sialylated structures are enclosed in brackets. Representative data from two independent analyses with similar tendencies are shown. A1, SLC35A1.