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. 2020 Dec 27;13(1):74. doi: 10.3390/polym13010074

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Evaluation of the release of biological markers into the culture media by human osteoblast spheroids after 7 days incubation inside the tested scaffolds. (A). Concentration of released Basic Fibroblastic Growth factor (FGF-2). (B). Concentration of released Vascular Endothelial Growth Factor (VEGF). (C) Concentration of released Platelet-Derived Growth Factor (PDGF). (D) Alkaline Phosphatase (ALP) activity. Bars indicate mean and standard deviation of three biological replicates with five technical replicated each. Bars with different letters are significantly different (p < 0.05). UE: units of enzyme activity.