Table 3.
An overview of released proteins showing significant differences between in vitro cultured MSC and osteoblasts. For more detailed information see Tables S6–S9).
| Increased in MSCs (n = 82) | Increased in Osteoblasts (n = 36) |
|---|---|
| Extracellular Matrix | |
| Collagens: COL1A1, COL1A2, COOL3A1, COL4A1, COL5A2, COL6A2, COL7A1, COL8A1, COL10A1, COL11A1, COL18A1 Others: ABI3BP, ECM1, EMILIN1, FBLN5, HAPLN1, HMCN1, NID1, POSTN (stem cell maintenance), TNC |
Collagens: COL2A1, Others: ACAN, EFEMp1, ELN, MXRA5 |
| Proteases, Protease Inhibitors | |
| Proteases: ADAM9, ADAM10, AEBP1, CTSC, FN1, MMP1, MMP11, PCOLCE (collagen), PLAU (coagulation) Inhibitors: SERPINB7 |
Proteases: CTSA, CTSD Inhibtor: SERPING1 |
| Other Enzymes | |
| ENPP1(phosphodiesterase), LOXL3 (lysyl oxidase), MAN1A1 (mannosidase), PTK7 (tyrosine kinase), SRPX (antioxidant) | C3 (complement), F10 (coagulation) |
| Protein Modulation/Stabilization | |
| CLU (chaperone), CTSC (chaperone), GRPEL1 (chaperone/HSP70) | HSPA6 (chaperon), SUMO2 |
| Extracellular Mediators | |
| CHID1 (growth factor, chemokine interaction), CLEC11A (growth factor), GREM1 (BMP antagonist), LTBP1 (TGF), MET (HGF receptor), TGFB1 (cytokine) | IGFALS, IGFBP3, NBL1 (BMP antagonist), PENK |
| Cell–matrix Adhesion | |
| ADAM10, CSPG4 (chondroitin), FN1, SDC1, TNC, VASP | SDCBP, THBS4 |
| Cell–cell Contact | |
| CCDC80 (cadherin), CDH11 (cadherin), CDH2 (cadherin), CHID1 (syndecan), HMCN1 (desmosomes), MCAM, PCDHGC3 (cadherin) | THBS4 |
| Cell Surface Molecules | |
| CD109, CDH11 (cadherin), CDH2 (cadherin), CHST11 (chondroitin), CSPG4 (chondroitin), NRP2 (semaphorin), SDC1, SEMA7A | VNN1 |
| Intracellular Transport, Endocytosis, Secretion | |
| ATP6AP1 (organellar acidification, V-ATPase), CHST11 (Golgi), CTSC (lysosome), GOLM1 (ER/Golgi), HSPA9 (ER/Golgi), MVP (nucleo-cytoplasmic), RRBP1 (ER), VAPA | RAB7A, SDCBP |
| Cytoskeleton | |
| ARPC3, MAPRE1, VAPA, VASP | MYH14, SDCBP |
| Protein Synthesis, Ribosome | |
| EIFRJ, EIF4B, RPL30, RRBP1 | PSMB8 (proteasome) |
| Intracellular Signaling | |
| CD109 (TGF), LTBP1 (TGF), MET (HGF receptor), MVP, PTK7 (Wnt) | CNPY2, PODN (Wnt), RAB7A (GTPase), SCUBE3 (TGFβ), RCSD1 (Wnt) |
| Angiogenesis | |
| CTHRC1, ECM1, FBLN5, NRP2 (VEGF) | |
| Metabolism | |
| ALDOC (glycolysis), GATD3A (mitochondria), GRPEL1 (mitochondria), HSPA9 (mitochondria), LDLR (lysosome, cholesterol), LIPA (lysosome, cholesterol), OCT1, mitochondria, ketones), PLTP (cholesterol), SDC1, TF (iron) | AHCY (adenosine, homocystein), APOC3 (lipid) |
| Transcription, RNA | |
| AEBP1, HNRNPUL2 (noncoding RNA), HSBP1 | PTMS, TSSK4 |
| Others | |
| ABI3BP (senescence, tumor suppressor), APEX1 (DNA repair, endonuclease), SRPX (antioxidant enzyme), SUMO1 (protein modulation) | BAX (apoptosis), HBa1 (hemoglobin), MT2 (antioxidant), SEPP (antioxidant) |
Abbreviations: BMP, bone morphogenic protein; ECM, extracellular matrix; ER, endoplasmatic reticulum; HGF, hepatocyte growth factor; TGF, transforming growth factor; VEGF, vascular endothelial growth factor; Wnt pathway, Wingless and Int-1 pathway.