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. 2020 Dec 28;22(1):210. doi: 10.3390/ijms22010210

Figure 4.

Figure 4

Effects of GHS-R1A antagonist (JMV2959) on WIN55,212-2-induced endocannabinoid and gamma-aminobutyric acid (GABA) extracellular changes in the rat NACSh. The JMV2959 3 mg/kg was injected i.p. 20 min before 2.4 mM/0.5 µL WIN55,212-2 or vehicle 0.5 µL administered into the posterior VTA injection (n = 6; means ± SEM). Changes in accumbens anandamide/N-arachidonoylethanolamine (AEA) levels are illustrated in graph (A), changes in 2-AG levels in the graph (B) and GABA changes are shown in graph (C). The effects are illustrated as follows: saline + WIN55,212-2 (filled circle; n = 7), 3 mg/kg JMV2959 + WIN55,212-2 (open circle; n = 6), 3 mg/kg JMV2959 + vehicle (open triangle; n = 4) and saline + vehicle (dotting; n = 7). Differences between saline + WIN55,212-2 and saline + vehicle groups are expressed as *** p < 0.001. Differences between JMV2959 + WIN55,212-2 and saline + vehicle groups are expressed as +++ p < 0.001, ++ p < 0.01. Differences between groups saline + WIN55,212-2 and 3 mg/kg JMV2959 + WIN55,212-2 are expressed as ### p < 0.001, # p < 0.05.