Figure 1.

A30 plants, overexpressing OsPYL/RCAR3, have reduced sensitivity to mannitol-imposed osmotic stress. (A–C), Seedling growth test. A8 and A30 seedlings were grown in growth medium alone (A) or containing mannitol (B) or NaCl (C). Scale bar: 2 cm. (D) Measurement of relative shoot length compared to each mock (0 mM). X axis indicates mannitol or NaCl concentrations, and growth period, (in d), in brackets. Two-way ANOVA was performed with A8 plants as controls. ****: p < 0.0001, -: not significant. (E) Effect of OsPYL/RCAR3 overexpression on luciferase activity from the OsRab16a promoter in rice protoplasts. Control: pRab16a: LUC alone. Data presented as mean ± standard deviation (SD), n = 3. Two-way ANOVA was performed, comparing with control. ****: p < 0.0001, -: not significant. OsPYL/RCAR3-HA expression was confirmed by immunoblot analysis with anti-hemagglutinin (HA) rat antibody.