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. 2021 Jan 1;22(1):393. doi: 10.3390/ijms22010393

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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OsPP2CAs interact with OsDREBs. (A), Venus C-terminus (VC)-tagged OsDREB1A, OsDREB1B and OsDREB1C were examined with Venus N-terminus (VN)-tagged OsPYL/RCAR3, OsPP2C06, OsPP2C09, and OsPP2C30 by BiFC analysis. Scale bars: 10 µm. (B), BiFC signal intensities normalized to internal control (ER-mCherry). Values are means ±SD (n > 20 cells) and normalized to BiFC signal for OsPYL/RCAR3-VN and each OsDREBs-VC. One-way ANOVAs were performed with comparing to signals interacted with OsPYL/RCAR3-VN (****: p < 0.0001, **: p < 0.01, -: not significant). (C), Co-immunoprecipitation analysis. Indicated constructs were introduced into rice protoplasts and GFP-tagged proteins were pull-downed with GFP-trap beads. An immunoblot analysis was performed with anti-GFP rabbit antibodies or anti-HA rat antibodies.