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. 2021 Jan 1;22(1):393. doi: 10.3390/ijms22010393

Figure 8.

Figure 8

Activation of pDRE::fLUC by overexpression of OsDREB1s. (A), Activation of pDRE::fLUC depends on OsDREB1s effector plasmid DNA amounts. One-way ANOVAs were performed with comparing to 0 μg of effector DNAs. (B), Confirmation of the lack of activation of the pABRE-DRE::fLUC reporter by OsPP2C09-i. One-way ANOVAs were performed, comparing to the control. (C), Effects of OsDREB1-mediated activation by OsPP2C09 or OsPP2C09-i. Two-way ANOVAs were performed with comparing to WT (OsPP2C09). (D), Effects of OsPP2C09 on the activity of OsDREB1A at two time points. Two-way ANOVA was performed, comparing to non-OsDREB1A. The indicated effector and marker DNAs were introduced into rice protoplasts by the PEG-mediated transfection method and then incubated for 15 h or 20 h. Induced LUC activity was detected with a dual-luciferase reporter assay system. All values of panels are means ± SD (n = 3). ****: p < 0.0001, **: p < 0.01, *: p < 0.05, -: not significant.