Figure 4.

Generation of the H460 COMMD1 KO cell line. (A) H460 WT and KO cell lines treated with CIGB-552 (25 µM) for indicated times and COMMD1, Bcl-2 and Bax were determined by Western blot analysis. Actin was used as a control for protein loading (B) CIGB-552 was added to 10,000 cells (0–200 μM) and incubated for 48 h. Cell viability was determined by sulforhodamine B sodium salt assay and the IC50 values were calculated. Data presented as the mean ± SD. * p < 0.05.