Figure 4.

PH aged females had higher cell proliferation compared to SI animals after stroke. (A) Representative images of the hippocampus of brain sections from mice administered bromodeoxyuridine (BrdU) (day 3 to 7) after stroke. Immunohistochemistry analysis and co-labeling with anti-BrdU (in Red) and DAPI (in blue) showed that mice that were isolated immediately after a stroke had lower BrdU-positive cells in the dentate gyrus. Magnified images in insert show co-labeling of BrdU and DAPI. Scale bar denotes 75 µm (B) Quantification of these BrdU-positive cells showed a significant increase in cell proliferation in the dentate gyrus of ST-PH mice compared to the ST-SI mice (n = 4/group. * p = 0.008). Data are presented as mean ± SEM. Student’s t-test was used for group comparisons.