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. 2020 Dec 28;10(1):78. doi: 10.3390/jcm10010078

Figure 2.

Figure 2

CTSB mediates pericellular proteolysis in hTM cells. (A) Representative image of pericellular proteolysis in hTM cells. Proteolytic degradation products of DQ-gelatin (green fluorescence) were monitored as described in materials and methods. (B) Representative image of pericellular proteolysis in hTM cells in the presence of the CTSB or cysteine cathepsin inhibitors Ca074Me and E64d, respectively. (C) Flow cytometry quantification of DQ-gelatin and DQ-Col I proteolytic degradation products in the presence and absence of Ca074Me, E64d, or marimastat (MMP inhibitor). Data are shown as the mean ± S.D. * p < 0.05; ** p < 0.01, n = 3. Hoechst (blue fluorescence) was used to stain nuclei. Red arrows represent the pericellular degradation of DQ-gelatin. Scale bars: 20 μm.