Figure 3.

Effect of AdoMet on cell cycle regulatory proteins in HCT 116^p53−/− and uL3ΔHCT 116^p53−/− colon cancer cells. The level of cell cycle-regulatory proteins in HCT 116^p53−/− (A) and uL3ΔHCT 116^p53−/− cells (B) was measured by western blot analysis. The housekeeping protein β-actin was used as a loading control. The relative densitometric analysis is reported in the corresponding graphs, expressed as arbitrary units. Bars represent the mean of triplicate experiments; error bars represent the standard deviation. * p < 0.05, ** p < 0.01 vs. untreated cells set at 1. The images are representative of three immunoblotting analyses obtained from three independent experiments. Full-length blots are shown in Figure S1.