Figure 6.

Effect of AdoMet on the autophagy related markers in HCT 116^p53−/− and uL3ΔHCT 116^p53−/− colon cancer cells. Representative immunoblotting of ATG7, p62, and LC3BI/II in HCT 116^p53−/− cells (A,C) and uL3ΔHCT 116^p53−/− cells (B,D) treated or not with 500 μM AdoMet for 48 h. The housekeeping protein β-actin was used as a loading control; graphs show the relative densitometric analyses, expressed as arbitrary units. Quantification of the LC3B-II/I ratio is shown in the graphs. Bars represent the mean of triplicate experiments; error bars represent the standard deviation. * p < 0.05, ** p < 0.01 vs. untreated cells set at 1. Full-length blots are shown in Figure S3.