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Sensitivity of the designed primers for three Chironex species (amplification curves). (a) 16S rRNA gene for Chironex Sp.A. (b) 16S rRNA gene for Chironex indrasaksajiae. (c) 16S rRNA gene for Chironex Sp.C. (d) COI gene for Chironex Sp.A. (e) COI gene for Chironex indrasaksajiae. (f) COI gene for Chironex Sp.C. 1: 10 ng; 2: 1 ng; 3: 0.1 ng; 4: and 0.01 ng (DNA template concentrations).
