Table 3.
List of the 16S rRNA primers of three Thai Chironex species.
| Sample | Name | Seq. (5’ → 3’) | Length (bp) | Tm (°C) | Product (bp) |
|---|---|---|---|---|---|
| Universal primer | P16sf | AAGGGCCGCGGTAACTCTG | 19 | 61.7 | 414 |
| S16sr | ACCCTGTTATCCCCGTGGT | 19 | 59.5 | ||
| Chironex Sp.A | P16sf | AAGGGCCGCGGTAACTCTG | 19 | 61.7 | 226 |
| CA16sr | ACCTGCTACTCCCTAAGGTTTAAATTTAGTG | 31 | 58 | ||
| Chironex indrasaksajiae | P16sf | AAGGGCCGCGGTAACTCTG | 19 | 61.7 | 226 |
| CI16sr | ACCTACTGTTCCCTAGAGTTTAAGTTTAAAGG | 32 | 57.6 | ||
| Chironex Sp.C | P16sf | AAGGGCCGCGGTAACTCTG | 19 | 61.7 | 217 |
| CC16sr | CCTCTAAAGTATCTAATCTAAAGTGGAGGGTAG | 33 | 57 |