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The concentration-dependent study of PP2Ac methylation status under 6,7-diprenoxycoumarin (50) treatment. Hep3B cells (1 × 106) were treated with 50 (5, 10, 20 and 40 μM) for 24 h. Treated cell lysates were prepared as described in the Methods section and methyl-PP2Ac expression was analyzed by western blot.
